大鼠脊髓急性损伤后血流动力学变化的光学监测

Observing hemodynamic changes in the spinal cord after acute spinal cord injury

目的观察脊髓损伤后血流动力学的变化，探讨脊髓损伤的血流动力学机制。方法雌性SD大鼠20只，分为对照组和损伤组，每组10只。Nystrom法制造大鼠脊髓（T10-11）中度压迫性损伤模型。利用激光散斑成像系统监测大鼠脊髓损伤后第10分钟、30分钟、1小时、2小时、3小时、6小时背部血管内的血流速度和血流量以及血管管径的变化。结果对照组大鼠各时间点血管内的血流速度、血流量以及血管管径均较稳定，无明显波动。损伤组大鼠各项指标均低于对照组。结论激光散斑成像技术可以用于监测脊髓血流动力学的变化；急性脊髓损伤后血流动力学的变化提示进行性的“创伤后缺血”，静脉回流受阻可能是引起创伤后缺血的一个重要因素。

Objective To observe any changes in spinal cord hemodynamics after spinal cord injury and to investigate their mechanism. Methods Twenty female Sprague-Dawley rats were divided into a control group and a moderate injury group, 10 rats in each group. A rat model of spinal cord injury caused by moderate compression was established using Nystrom＇s method. Changes in the velocity and volume of blood flow and in the diameters of vessels of the spinal cord were monitored for 6 hours using laser speckle imaging. Results No changes in the velocity or volume of blood flow or in the diameter of vessels were observed in the control group, but all of these indices diminished significantly in the injury group. Conclusions Laser speckle imaging can be used to monitor changes in spinal cord hemodynamics. Such changes after acute spinal cord injury indicate progressive post-injury ischemia. The occlusion of veins may be an important contributor to post-injury ischemia.