局部湿热联合微波消融对乳腺癌荷瘤小鼠肿瘤生长和血清干扰素-γ白细胞介素-12的影响

The effects of local wet hyperthermia combined with microwave ablation on tumor growth and serum levels of interferon-β and interleukine-12 in mouse models of mammary carcinoma

目的研究局部湿热联合微波消融对肿瘤生长和血清干扰素-γ（IFN-γ）、白细胞介素-12（IL-12）的影响，并探讨其意义。方法48只Balb／c小鼠随机分为荷瘤未处理组、微波消融组、局部湿热组、微波消融＋局部湿热组，每组12只。先建立小鼠乳腺癌实验动物模型，14d后进行微波消融处理，再过7d后进行局部湿热处理，每周1次，共2次。观察各组肿瘤生长情况、生存情况和肺转移情况，并用酶联免疫吸附法检测血清IFN-γ、IL-12的变化。结果微波消融＋局部湿热组的肿瘤生长大小和肺转移指数明显低于微波消融组和局部湿热组（P〈0．05），生存时间明显延长（P〈0．05），血清IFN-γ和IL—12的浓度也显著增加（P〈0．05）。结论局部湿热联合微波消融可以较单一热疗方法更有效地抑制肿瘤生长，这可能与其免疫反应的明显增强有关。

Objective To investigate the effects of local wet hyperthermia combined with microwave ablation on tumor growth and serum levels of interferon-β （IFN-β） and interleukine-12 （IL-12）. Methods An experimental mouse model bearing mammary carcinoma was established in Balb/c mice and then treated with microwave ablation after 14 d. Seven d after ablation, local wet hyperthermia was administered once weekly for 2 weeks. Tumor growth and lung metastasis, as well as the survival of bearing tumor mice were observed, The serum levels of IFN-β and IL-12 were detected with enzyme-linked immuno sorbent assay （ELISA）. Results When local wet hyperthermia was combined with microwave ablation tumor growth and lung metastasis were significantly less than when microwave ablation or local wet hyperthermia was used alone （P 〈 0.05 ）. Furthermore, the survival of bearing tumor mice in combined therapy group prolonged significantly compared with those receiving either therapy alone （P 〈 0.05 ）. Serum levels of IFN-β and IL-12 were also significantly higher in combined therapy group compared with the other groups （P 〈 0.05）. Conclusions Local wet hyperthermia combined with microwave ablation is more effective than either local wet hypertbermia or microwave ablation alone in retarding tumor growth, which may be related to the enhanced immune response.