模拟高原低氧环境下大鼠牙周炎致病菌多样性的实验研究

The pathogenic bacteria diversity of rat periodontitis in imitative plateau environment: an experimental study

目的:建立大鼠高原牙周炎动物模型,通过检测其牙周炎临床指标和龈沟液中菌群多样性,探讨高原牙周炎的发病机制。方法:80只SD大鼠随机分为平原对照组、平原结扎组、高原对照组、高原结扎组。结扎组采用正畸结扎丝结扎上颌第一磨牙,各组均给予高糖饮食,高原组置低压氧舱饲养,于8周后检测第一磨牙牙周炎临床指标,提取龈沟液细菌总DNA,使用PCR-变形梯度凝胶电泳技术(Polym erase chain reaction-denaturing grad ient gel electrophoresis,PCR-DGGE)分析龈沟液中牙周炎菌群差异性,选择特异性DNA条带,回收测序并进行序列分析。结果:与平原结扎组相比,高原结扎组的牙龈指数、牙周袋深度和牙松动度均有显著统计学差异(P<0.05);高原组与平原组龈沟液的菌群的基因型分布之间具有统计学差异(P<0.05);测序结果表明高原结扎组和平原结扎组优势菌不尽相同。结论:成功建立了大鼠高原牙周炎动物模型,其龈沟液中牙周炎致病菌的多样性与平原牙周炎相比具有显著差异。

AIM ： To study the pathogenesis of plateau periodontitis, by creating a rat model of experimental periodontitis in plateau environment and detecting the bacteria diversity in gingival crevicular fluid. METHODS： Eighty SD rats were divided into the plain control group, plain ligated group, plateau control group and plateau ligated group. Maxillary first molars of the ligated group were ligated by steel ligature, the plateau groups were bred in low pressure oxygen granary. The clinical indexes were detected after 8 weeks. Total DNAs were extracted from the bacteria in gingival crevicular fluid, and the diversity of pathogenic bacteria was detected by the PCR - DGGE analysis method. Bands were sequenced, and the sequences of bands were analyzed. RESULTS： Gingival index, the depth of pocket and tooth mobility of the first maxillary molar with periodontitis in the plateau ligated group were more serious than those in the plain ligated group（ P 〈 0.05 ）. The diversity of pathogenic bacteria in various groups was statistically different （ P 〈 0.05 ）. The retrived sequences showed that the predominance bacteria of plateau ligated group were different from the plain ligated group. CONCLUSION： The rat plateau periodontitis can be established successfully, and the diversity of pathogenic bacteria is present between the plateau periodontitis and plain periodontitis.