大黄酸对转化生长因子β1诱导的人肾小管上皮细胞血小板反应蛋白1表达的影响

Effect of rhein on expression of thrombospondin-1 in renal tubular cells induced by transforming growth factor β1

目的探讨大黄酸对转化生长因子β1（transforming growth factor β1，TGF-β1）诱导的人肾小管上皮细胞（HK-2）血小板反应蛋白1（thrombospondin-1，TSP1）表达的影响。方法体外培养细胞，观察TGF-β1（4ng／ml）诱导下低、中、高浓度的大黄酸（10μg／ml、20μg／ml和40μg／ml）对HK-2细胞TSP1 mRNA和蛋白表达的影响。Real Time PCR检测TSP1 mRNA表达，Western Blot检测TSPI蛋白表达。结果与空白对照组比较，TGF-β1（4ng／ml）明显上调HK-2细胞TSP1 mRNA和蛋白表达。与TGF-β1（4ng／ml）阳性对照组比较，中浓度和高浓度大黄酸明显抑制了TSP1 mRNA和蛋白表达。结论大黄酸能抑制TGF-β1诱导的HK-2TSP1 mRNA和蛋白表达。

Objective To investigate the effect of rhein on the expression of thrombospondin-1 in renal tubular cells induced by transforming growth factor β1. Method HK-2 cells were stimulated by different concentrations of rhein（10,20, and 40 μg/mi） in the presence of TGF-β1 （4 ng/ml）, untreated HK-2 cells served as normal control group,and HK-2 cells stimulated by TGF-β1 （4 ng/ml） as positive group. Real Time PCR and Western Blot were used to detect the expression of TSPI mRNA and protein,respectively. Result The expression of TSP1 mRNA was up-regulated obviously in HK-2 cells when stimulated by TGF-β1 （4 ng/ml）. The protein expression of TSP1 was also up-regulated simultaneously in HK-2 cells when stimulated by TGF-β1 （4 ng/ml）. The expression of TSP1 mRNA and protein induced by transforming growth factor 61 was down-regulated markedly with 20 μg/ml and 40 μg/ml of rhein. Conclusion Rhein can inhibit the expression of TSP1 mRNA and protein.