嗅鞘细胞经低能激光照射后移植对大鼠脊髓损伤神经功能修复的影响

Transplantation of olfactory ensheathing cells irradiated by low power laser irradiation to repair spinal cord injury in rats

目的探讨嗅鞘细胞（OECs）经低能激光照射（IPLI）后移植对大鼠脊髓横断损伤后功能修复的影响。方法24只SD大鼠T12脊髓完全横断伤模型建模4周后，随机分为OECs移植组、经LPLI的OECs的移植组和空白对照组（DMEM培养液），应用BBB评分法、组织学评价以及Flurogold逆行示踪评价各组脊髓损伤的修复情况。结果三组大鼠不同移植方式以及评分时间点对移植后下肢功能BBB评分结果的影响差异均有统计学意义（P=0．000），且组间差异均有统计学意义（P=0．000）。经LPLI的OECs移植组大鼠头尾侧均可见NGFRp75阳性及GFAP阳性的OECs，OECs移植组则仅可见GFAP阳性的OECs，空白对照组未见NGFRp75阳性及GFAP阳性的OECs。OECs移植组和经LPLI的OECs移植组大鼠头尾侧及瘢痕区均可见Flurogold标记的神经纤维穿行，空白对照组未见Flurogold标记的神经纤维穿行。结论OECs及经LPLI的OECs均可促进损伤脊髓的功能恢复，OECs经LPLI后可能更具有促进损伤脊髓功能恢复的作用。

Objective To investigate the effects of transplantation of olfactory ensheathing cells （OECs） irradiated by low power laser irradiation （LPLI） on the repair of transversal spinal cord injury in rats. Methods Twenty-four SD rats were randomized into 3 even groups after the animal models of spinal cord completely transected at T12 had been established for 4 weeks. OECs（group A）, OECs irradiated by LPLI （group B ） and DMEM fluid （empty control group ） were transplanted into the caudal zone of the spinal cord in the 3 groups （n = 8 ） respectively. The functional repair was evaluated by Bundle branch bloek（BBB） score, pathology and Flurogold label, respectively. The BBB scores before and after transplantation were statistically analyzed by one-factor analysis of variance or repeated measurement analysis of variance, respectively （ a = 0. 05）. Results The methods of transplantation and the evaluation time had significant effects on the BBB scores in the 3 groups （ P = 0. 000）, and the BBB scores were significantly different among the 3 groups （ P = 0. 000）. Anti-NGRFp75 and anti-GFAP staining positive OECs were observed in the cephalic and caudal areas in group B, only anti-GFAP staining positive OECs were observed in group A, but nothing was found in the empty control group. The neural fibers labeled by Flurogold passed through the lesion area and extended into the cephalic and caudal areas in groups A and B, but were not present in the empty control group. Conclusion Although transplantation with both OECs and OECs irradiated by LPLI can promote the repair of spinal cord injury in rats, OECs irradiated by LPLI may be more effective.