HPLC-ELSD法测定参银颗粒中人参皂苷Rg1及Re的含量

Determination of Ginsenoside Rg1 and Ginsenoside Re in Shenyin Granules by HPLC - ELSD

目的:用HPLC-ELSD法测定参银颗粒中人参皂苷Rg1及Re的含量。方法:色谱柱填料为十八烷基键合硅胶,流动相为乙腈-水;温度40℃,气体压力3.5bar。结果:人参皂苷Rg1及Re分别在0.9012～9.012,1.219～12.19μg之间呈良好的线性关系;参银颗粒中2种成分的平均回收率分别为98.2%(RSD 1.3%),97.8%(RSD1.5%);结论:该方法简便、准确、分离效果好,无干扰,可用于参银颗粒的质量评价。

Objective：To establish the quantitative method of ginsenoside Rg 1 and Re in Shenyin granules by HPLC - ELSD. Methods：The column was packed with 5μm Diamonsil C18 stationary phase. The mobile phase consisted of acetonitrile -water. The temperature was 40 ℃ and the pressure was 3.5bar. Results：The linear ranges of the two components were 0.9012 - 9. 012μg,1. 219 - 12. 19μg.