川芎提取物的含量标准研究

Determination of contentstandard for rhizoma of chuanxiong extract

目的:建立川芎提取液的含量标准控制方法。方法:采用高效液相色谱法测定阿魏酸的含量以确定相关提取物的含量。用十八烷基键合硅胶为填充剂;以甲醇-1%冰醋酸溶液(35:65)为流动相;检测波长为280nm;理论塔板数按阿魏酸峰计算应不低于4000。结果:阿魏酸在0.0505～0.404ug(R=0.9996)范围有良好的线性关系,平均回收率为98.1%,RSD=0.53%(n=9)。结论:以阿魏酸对川芎提取物进行质量标准控制的方法,适合用于川芎提取物的质量标准控制。

Objective： To establish the quality standard control methodology of determining the contents of chuanxiong extract. Methods： Ferulic acid what is determined hy HPLC is used to determine the amount of related extract. An YMC - C18 column was used with a solvent system consisting of MeOH - 1% HAc （ 35 ： 65 ）, and the wavelength of detection was set at 280nm to determine the ferulic acid. Results：Tbe operating curves wee found to be linear over the ranges of 0. 0505 -0. 404ug （R=0.9996）. The recovery was98. 1%, RSD =0.53% （n =9）. Conclusion：The method can be used for the quality standard control of ferulic acid.