大豆异黄酮对小鼠子宫内膜异位症退化作用的研究

Study on the regression effect of soy isoflavone on endometriosis of rats

目的:探讨大豆异黄酮对小鼠子宫内膜异位症(Endometriosis,EMs)的退化作用。方法:制备EMs小鼠模型,分为对照组(A组)、模型组(B组)、大豆异黄酮低(C组)、中(D组)、高(E组)剂量组。观察治疗前、后异位病灶体积并做病理检查,采用ELISA法检测白细胞介素-1β(Interleukin-1β,IL-1β)和肿瘤坏死因子-α(Tumour necrosis factor-alpha,TNF-α)含量。结果:该实验小鼠子宫内膜异位模型的形成率为73.3%。C组治疗前、后移植物体积无明显差异(P>0.05)。D组和E组治疗前、后移植物体积比较差异有统计学意义(P<0.05)。B组血清中IL-1β和TNF-α的含量明显高于A组(P<0.05),C组与B组比较无明显差异(P>0.05)。D、E组IL-1β和TNF-α的含量均显著低于B组(P<0.05)。结论:大豆异黄酮对小鼠内膜异位移植物有明显的退化作用,其机制可能与大豆异黄酮抑制雌激素受体的活性,降低血清IL-1β和TNF-α的含量有关。

Objective： To explore the regression effect of soy isoflavone on endometriosis of rats. Methods ： Rat models of endometriosis were established and divided into control group （ group A ）, model group （ group B ）, low - dose soy isoflavone group （ group C）, middle - dose soy isoflavone group （ group D） and high - dose soy isoflavone group （ group E） . The volumes of ectopic lesions before and after treatment were observed and pathologic examination was conducted. The levels of interleukin -1β（ IL - -1β） and tumor necrosis factor -α（TNF -α） were detected by ELISA. Results： The success rate of model establishment was 73.3% in the study. There was no significant difference in implants volume before and after treatment in group C （ P 〉 0. 05 ） . There was significant difference in implants volume before and after treatment in group D and group E （ P 〈 0.05 ） . The serum levels of IL-1β and TNF -α in group B were significantly higher than those in group A （ P 〈0. 05 ）, but there was no difference between group C and group B （ P 〉 0.05 ） . The serum levels of IL -1β and TNF -α in group D and group E were significantly lower than those in group B （P 〈 0. 05 ） . Conclusion： Soy isoflavone can cause a significant regression of endometriotic implants, and the mechanism may be related to the suppressive effect of soy isoflavone on the activity of estrogen receptor and serum levels of IL-1β and TNF -α.