摘要
重组大肠杆菌BL21/pET22b-argE表达的N-乙酰鸟氨酸脱酰基酶(NAOase)可用于脂肪族氨基酸的手性拆分,水解N-乙酰-D,L-氨基酸中的L-型底物,N-乙酰-D,L-蛋氨酸为其最合适的底物。为了确定NAOase拆分N-乙酰-D,L-蛋氨酸合适的转化条件,考察了反应温度、pH值、Co^(2+)浓度、转化时间、底物浓度和加酶量对产物的影响。结果表明,合适的反应条件为37℃,pH值7.0,Co^(2+1) mmol/L,转化时间20 min,底物浓度150 mmol/L,菌泥5 g/L。在上述反应条件下,N-乙酰-D,L-蛋氨酸的转化率可达81.4%。
N-acety-L-ornithine deacetylase （NAOase） expressed by recombinant engineering strain BL21/pET22b-argE can be used to separate chiral aliphatic amino acids, and its optimal substrate is N-acetyl-D,L-methionine. In order to obtain the appropriate conversion conditions of N-acetyl-D,L -methionine by NAOase, the effects of temperature, pH, concentration of Co^2＋, concentration of substrate and enzyme dosage on conversion of N-acetyl-D,L-methionine were investigated. The results showed that the conversion of N-acetyl-D,L conditions of 37 ℃, pH7.0, concentration of Co^2＋ 1 of substrate 150 mmol/L and enzyme 5 g/L.
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
2009年第4期329-333,共5页
Chemical Reaction Engineering and Technology
基金
国家973项目基金资助(2009CB724702)
关键词
N-乙酰鸟氨酸脱酰基酶
N-乙酰-D
L-蛋氨酸
酶法拆分
转化条件
methionine was 81.4% under the appropriate mmol/L, reaction time 20 min, concentration N-acety-L-ornithine deacetylase
N-acetyl-D, L-methionine
enzymatic resolution
operation condition
