健脾益气中药对脾虚大鼠肝组织线粒体功能的影响

Effect of Spleen-invigorating and Qi-tonifying Herbs on Hepatic Mitochondrial Function in Rats with Spleen Asthenia

目的观察黄芪四君子汤和四君子汤对脾虚大鼠肝组织线粒体琥珀酸脱氢酶、细胞色素氧化酶活性的影响,从能量代谢角度揭示脾虚证的发生机理,阐明健脾益气中药的作用机制。方法取SD大鼠40只,随机分为4组,即空白对照组、脾虚模型组、四君子汤组(10.5g/kg)和黄芪四君子汤组(18g/kg),每组10只,空白对照组与脾虚模型组给予等体积的生理盐水,每日1次,连续4周。除空白对照组外,其余各组大鼠以破气苦降加饥饱失常的方法建立大鼠脾虚模型,每日灌饲小承气汤煎剂60g/kg,隔日半量进食,自由饮水,造模15d后,开始灌胃给予相应的药物。观察各组大鼠的一般情况,以及肝组织线粒体琥珀酸脱氢酶和细胞色素氧化酶活性的变化情况。结果与空白对照组比较,脾虚模型组大鼠肝组织线粒体琥珀酸脱氢酶、细胞色素氧化酶活性均明显下降(P<0.01);黄芪四君子汤和四君子汤均可显著提高脾虚大鼠肝组织线粒体琥珀酸脱氢酶和细胞色素氧化酶的活性(P<0.01),且黄芪四君子汤的疗效优于四君子汤。结论健脾益气中药能够明显地改善脾虚大鼠肝组织线粒体的损伤和能量代谢的障碍,黄芪四君子汤的疗效优于四君子汤。

Objective To observe the effect of Sijunzi Decoction （SD） and Huangqi Sijunzi Decoction （HSD） on mitochondrial succinate dehydrogenase, cytochrome oxidase activity of liver in rats with spleen asthenia, thus to reveal the pathogenesis mechanism of spleen asthenia from energy metabolism, and to clarify the therapeutic mechanism of spleen -invigorating and Qi-tonifying herbs. Methods Forty SD rats were randomly divided into 4 groups： normal control group, spleen - asthenia model group, SD （10.5 g/kg） group and HSD （18 g/kg） group, 10 rats in each group. Except that the normal control group, the rats in the other groups were fed with half - full diet once every other day and with gastic garage of Xiaochengqi Decoction 60 g/kg every day to establish animal model of spleen asthenia. After 15 days, the model rats were administered the corresponding drugs according to the experimental design for 4 continuous weeks. The changes of mito- chondrial suceinate dehydrogenase, cytoehrome oxidase activity of liver were measured, and the general status of the rats were observed. Results Compared with the normal control group, the level of mitochondrial succinate dehydrogenase and cytochrome oxidase activity in liver of spleen asthenia group were decreased obviously （ P 〈 0. 01 ） ; While SD and HSD enhanced the level of mitochondrial succinate dehydrogenase and cytochrome oxidase activity in liver （ P 〈0. 01 compared with the model group）, and the effect of HSD was better. Conclusion Herbs wtih the actions of invigorating the spleen and tonilying Qi can relieve obviously the mitochondrial injury and the obstacle of energy metabolism in liver mitochondria of spleen asthenia rats, and the effect of HSD is better than that od SD.