Co^(60)诱变管囊酵母发酵木糖产乙醇的研究

Strain Improvement of Pachysolen tannophilus by Co^(60) Mutagenesis for Xylose Alcoholic Fermentation

通过Co60诱变使管囊酵母利用木糖发生突变。经在纯木糖培养基上的筛选,分离得到有较高木糖利用效率和乙醇发酵效率的诱变菌株800-3,考察了发酵条件对其产乙醇的影响。结果证明,溶氧量和培养基组分对于乙醇的产率有重要影响,在转速100 r/min,100 mL三角烧瓶中装液20 mL,酵母浸膏浓度1 g/L条件下,发酵液中的乙醇质量浓度只有5.86 g/L,而木糖醇质量浓度可以达到12.95 g/L,乙醇产率与前人的研究结果相比还有较大差距。

Xylose-fermenting yeast Pachysolen tannophilus was mutated by Co^60 rays. The mutant 800-3, which shows superior xylose utilizing and ethanol producing efficiency was isolated after selection. The effects of fermentation conditions on ethanol production were investigated. It is shown that the dissolved oxygen level and culture medium components exhibit significant effects on the ethanol yield. Under the conditions of shaking rate 100 r/min, medium volume 20/100 mL and YE concentration 1 g/L, the concentration of ethanol is 5.86 g/L, while that of xylitol is 12.95 g/L. Comparing with the former studies, the ethanol yield is much lower.