油菜抗羟脯氨酸突变体的筛选

SELECTIONS OF THE MUTANT RESISTANT TO HYDROXYPROLINEIN BRASSICA NAPUS

本文用脯氨酸的类似物羟脯氨酸筛选出经甲基磺酸乙酯（ＥＭＳ）诱变处理的油菜幼胚培养苗的抗羟脯氨酸突变体．抗性系叶片中的绝大多数游离氨基酸的含量都比对照品种“Ｏｒｏ”高，其中游离脯氨酸的含量是“Ｏｒｏ”的２２倍，表明用羟脯氨酸筛选出的突变体植株细胞内的游离氨基酸，特别是游离脯氨酸的含量明显增加．抗性系后代的多种农艺性状试验结果表明，抗性系的产量比对照高３１１％，发病率低３８５％，病情指数下降００９％。

The mutant of Brassica napus resistant to hydroxyproline was obtained through embryo culture in combination with mutagenic treatment by EMS(ethylmethane sulfonate),and through selection by hydroxyprolineThe contents of the most of the free amino acids in the leaves of the resistant strain were higher than those of the control,and the content of the free proline of the resistant strain was 22 time as many as that of the controlThis result indicated that the contents of the free amino acids,especially the free proline,were increased obviously by this methodExperiments and observations of various agronomic characteristics of the resis-tant strain showed that its yield was 311% higher the incidence of Sclerotinia sclerotiorum was 385% lower than those of the control Based on the above results,it is suggested that selection of the mutant resistant to hydroxyproline is an effective method in the breeding of Brassica napus