摘要
采用ＲＡＰＤ分析方法对５个番茄品种的基因组进行了检测．结果表明，随机引物以１０个核苷酸为宜，最佳ＰＣＲ反应条件为９４℃变性３０ｓ，３６℃退火６０ｓ，７２℃链延长９０ｓ．在该反应条件下，检测了５个番茄品种（系）和一个杂一代的基因组ＤＮＡ，每个样品可产生多条扩增片段，在不同的品种间表现出明显的多态性．
Genomes of 5 varieties of tomato( L.esculentum ) were tested by using the RAPD method.The result showed that suitable length of random primer was 10 mer and the optimum PCR condition was: denature at 94℃ for 30s, annealing at 36℃ for 60s and chain elongation at 72℃ for 90s.Genomes of 5 varieties and 1 hybrid F 1 tested under this condition showed great polymorphism among varieties, each of which can produce many amplifying band.
出处
《内蒙古师范大学学报（自然科学汉文版）》
CAS
1998年第3期223-226,共4页
Journal of Inner Mongolia Normal University(Natural Science Edition)
基金
内蒙古自然科学基金
