高效液相色谱法测定血浆中小蘖硷浓度(英)

Determination of Berberine Concentrationin in plasma by HPLC

研究了心衰病人口服小蘖硷后的吸收性，血浆样品用甲醇预处理以沉淀其蛋白质，小蘖硷测定条件为：μBondapakC18柱（10μm，300×3．9mmi．d．）乙腈和0．02mol／L磷酸混合液（45：55．v／v）为流动相，紫外鉴定器设定在波长为346nm处，进样量为50μl时，最小检测极限为8ng／ml。小蘖硷平均回收率为92．5％，结果表明本试验建立的方法和血浆中黄连素浓度监测有利于指导治疗心衰。

Berberine absorption by patients with ischemic heart failure (lHF) after oral administrationawas studied. Plasma samples were pretreated by protein precipitation with methanol. Berberinewas determined on a μBondapak column (10μm, 3OO×3. 9 mm i. d. ) with acetonitrile and 0.02mol/L phosphoric acid (45: 55, v/v)mixture as mobile phase. UV wavelength was set at 346nm. The detection limit for berberine in plasma was 8 ng/ml for an injection volume of 50μl. Theaverage berberine recovery was 92. 5%. Results showed that the method established in this ex-periment and plasma berberine monitoring may be helpful in the treatment of patients with IHF.