阵列叉指式芯片研究细胞介电电泳富集过程

Study of Cell Enrichment Process by Dielectrophoresis on Dielectrophoresis Microchip with Interdigitated Arrayed Electrodes

采用阵列叉指电极介电电泳(Dielectrophoresis,DEP)芯片,构建了集成DEP芯片分析和操控系统,应用Coventorware有限元分析软件模拟分析了芯片表面的电场分布情况;以红细胞和结肠癌细胞样品为分析对象,实现了两种细胞样品在芯片上的正负介电电泳定位富集。实验发现,交流信号幅值Vp-p是决定DEP富集效率的主因,交流信号频率f和缓冲溶液是改变细胞介电电泳类型的参量;在0.9%NaCl中,施加频率为10和3MHz、电压5V的交流频率,结肠癌细胞的正介电电泳(Positive-dielectrophoresis,pDEP)和负介电电泳(Nagetive-dielectrophoresis,nDEP)富集效率分别为87.2%和84.8%。

An integrated dielectrophoresis（DEP） microchip system was constructed using DEP microchip with interdigitated arrayed electrodes,and electric field distribution at different heights avove the electrode surface were simulated and discussed in Coventorware finite element analysis software.Different types DEP positioning enrichment of the red blood cells and colon cancer cells was achieved on the constructed system.The DEP enrichment and manipulation parameters were optimized according to the peak value of applied AC signal and its frequency,as well as the type and composition of buffer solution.With the optimized operation parameters of AC signal peak value at 5 V,the frequency of AC signal 10 MHz or 3 MHz and 0.9% physiological saline buffer solution,the enrichment efficiency of positive and negative DEP to colon cancer cells reached 87.2% and 84.8%,respectively.