二维纳米金单层膜的构建及其生物电化学应用

Preparation of Two-dimensional Nano-gold Particles Monolayer and Its Application to Bioelectrochemistry

利用Langmuir-Blodgett(LB)技术在氧化铟锡(ITO)电极上制备了二维纳米金(nano-Au)单层膜,采用扫描电子显微镜表征了二维纳米金单层膜。实验结果表明:表面压为28mN/m时,可获得分散性好、形状规则且分布均匀的二维球形纳米金单层膜。利用LB技术制备了肌红蛋白(Mb)薄膜,并将其固定在二维纳米金单层膜修饰的电极表面,研究了肌红蛋白LB膜的直接电化学行为。结果表明:纳米金粒子能够有效地加速肌红蛋白的电子转移,其电子转移速率为1.415s-1。

By the Langmuir-Blodgett technique,two-dimensional nano-gold monolayers were prepared on indium-tin oxide electrodes.The nano-gold monolayers were investigated using SEM.The experimental results showed that the nano-gold monolayers are dispersive,regular and even at the surface pressure of 28 mN/m.The myoglobin membranes fabricated by the Langmuir-Blodgett technique were immobilized onto the electrodes modified with two-dimensional nano-gold monolayers.Direct electrochemistry of the myoglobin was studied.The results indicated that the nano-gold particles could efficiently accelerate the electron transfer of myoglobin and the electron transfer rate was calculated to be 1.415 s-1.