用SSR分子标记研究茶组植物种间亲缘进化关系

Phylogenetic Analysis of Interspecies in Section Thea Through SSR Markers

本研究利用50对来自茶系的SSR引物对41份茶组资源进行PCR扩增,研究不同类型SSR引物的通用性和多样性指数。研究结果表明,茶系的SSR引物在茶组植物中的通用性高,其中EST-SSR通用性多态位点的比例达到60%。另外,利用SSR标记技术对茶组材料的遗传多样性进行分析。不同位点在种间的等位基因数为2～6个,平均每个位点4.21个等位基因。通过UPGMA方法构建了茶组12个种的遗传进化关系图。

For the purpose of investigating the transferability and polymorphism information content （PIC） of different types of SSR primers, PCR amplification for 41 accessions from section thea was conducted in this investigation. The results showed that the SSR primers form Ser. Sinensis were highly transferable among section Thea. The polymorphic and transferable primers account for 60% of EST-SSR markers. Then the genetic diversity among 12 species of section Thea was evaluated through SSR analysis. The number of alleles in different loci ranged from 2 to 6, with average 4.21 per locus. The phyolgenetic dendrogram was constructed by the UPGMA.