ISSR标记鉴别云南茶树种质资源的研究

ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province

利用最少数量引物获得最大鉴定能力,这对种质资源的分子鉴别尤为重要。本研究应用ISSR标记技术对134份云南茶树资源进行了分子鉴别研究,用3种独立的方法对茶树种质资源进行了鉴别:特殊标记、特异的谱带类型和不同引物提供谱带类型组合。结果表明,UBC807等12个引物扩增的10个特异标记的存在和15个特异标记的缺失,可以为香竹箐大山茶等21份资源提供鉴别依据。引物UBC811扩增的54种谱带类型可以为海南大叶茶1等35份资源提供鉴别依据。UBC811、UBC835、ISSR2、ISSR3等4个引物带型的组合可以鉴别所有的134份茶树资源,并为这134份云南茶树资源的构建了可以相互区别的DNA指纹图谱。

It was of much importance using minimum primers to obtain the maximum identification ability. Molecular identification of 134 tea germplasm resources using inter-simple sequence repeat （ISSR） markers was conducted in this study. There were 3 independent ways to identify tea germplasms： a） unique ISSR markers： b） unique band patterns and c） a combination of the band patterns provided by different primers. The result showed that the presence of 10 unique ISSR markers and the absence of 15 unique markers obtained from 12 primers made it possible to identify 21 tea germplasms, including ＇Xiangzhuqingdashancha＇ and so on. Using 54 unique band patterns of primer UBC811 could identify 35 tea germplasms, including ＇Hainandayecha 1＇ and so on. All the 134 tea germplasms could be entirely identified by the band patterns combination of primer UBC811, UBC835, ISSR2 and ISSR3, which was successfully used to construct the ISSR fingerprinting for discriminating 134 tea germplasms from Yunnan Province.