TGF-β_1抑制剂灯盏花素对肿瘤诱导的小鼠免疫抑制的逆转作用

Reversal of tumor induced immunosuppression by breviscapine in mice

目的:观察灯盏花素对肿瘤诱导的小鼠免疫抑制的影响。方法:小鼠脾脏细胞体外培养观察灯盏花素对淋巴细胞增殖和淋巴细胞因子分泌的的影响;lewis肺癌荷瘤小鼠迟发型变态反应、血清淋巴细胞因子水平及CD8+T细胞对lewis肺癌的细胞毒作用检测灯盏花素对小鼠免疫抑制的逆转作用。结果:灯盏花素体外对刀豆蛋白(ConA)诱导的小鼠脾脏细胞增殖有增强作用,能抑制转化生长因子β1(TGF-β1)和白细胞介素4(IL-4),促进的干扰素λ(IFN-λ)和白细胞介素2(IL-2)。体内灯盏花素能逆转荷瘤小鼠迟发型变态反应、血清淋巴细胞因子水平及CD8+T细胞对lewis肺癌的无能作用,并一定程度抑制肿瘤生长。结论:灯盏花素作为TGF-β1抑制剂对肿瘤诱导的小鼠免疫抑制有逆转作用。

Objective： To observe effects of breviscapine tumor induced immunosuppression by breviscapine in mice. Methods： The effects of breviscapine on lymphocyte proliferation and cytokines secretion were examined by cultured spleen cells in vitro. The reversal of tumor induced immunosuppression was assessed by delayed type hypersensitivity, lymphocyte factors level and CD8＋cytotoxicity against lewis lung cancer cells in mice bearing lewis lung cancer. Results： In vitro, breviscapine could enhance lymphocyte proliferation induced by ConA, inhibit TGF-β1and IL-4 , promote IFN-λ, and IL 2. In vivo, breviscapine could reverse delayed type hypersensitivity, lymphocyte factors level and CD8＋T cell anergy against lewis lung cancer cells in mice bearing lewis lung cancer. Conclusion： Breviscapine as a TGF-β1 inhibitor could reverse tumor-induced immunosuppression in mice.