灯盏花素对小鼠乳腺癌术后转移和复发的预防作用

Preventive effect of breviscapine on metastasis and recurrence of breast cancer after the tumor removal in mice

目的:观察灯盏花素对小鼠乳腺癌手术切除后转移和复发的影响。方法:将小鼠4 T1乳腺癌细胞植入雌性BALB/c小鼠乳房脂肪垫下。乳腺癌直径达10 mm时手术切除,次日治疗开始,包括灯盏花素治疗、阿霉素化疗及放射治疗。治疗效果通过小鼠术后转移和复发率、小鼠血清干扰素λ(IFN-λ)和白细胞介素2(IL-2)、及小鼠存活时间评价。结果:对照组、阿霉素化疗组、放射治疗和灯盏花素组乳腺癌复发率分别为50%、100%、100%和20%;肺内出现转移灶率分别为70%、100%、100%和20%。与对照组比较,阿霉素化疗组和放射治疗组小鼠有明显较低的血清IFN-λ和IL-2,而灯盏花素组小鼠血清IFN-λ和IL-2均升高。结论:乳腺癌手术切除后机体的免疫功能恢复对控制转移和复发十分重要。灯盏花素能促进机体免疫功能的恢复,因此可以用于预防乳腺癌术后转移和复发。

Objective： To investigate the effect of breviseapine on metastasis and recurrence of breast caneer（4T1） after the tumor removal in mice. Methods： 4T1 breast cancer cells were injected into the second mammary fat pad of BALB/c mice. The breast cancer was removed by operation when it reached a diameter of 10mm, next day, the treatment was initiated, including breviscapine treatment, doxorubicin chemotherapy and radiotherapy. The therapeutic efficacy was evaluated by the rate of metastasis and recurrence, serum IFNλand IL 2, and life span in mice. Results： The rates of tumor recurrence were 50% , 100%, 100% and 20% in the control, doxorubicin chemotherapy, radiotherapy and breviscapine groups, respectively. The pulmonary metastasis rates were 70% , 100 % , 100% and 20% in control, doxorubicin chemotherapy, radiotherapy and breviscapine group, respectively. Compared with the control group, serum IFN-λand IL-2 significantly decreased in doxoruhiein chemotherapy and radiotherapy groups, whereas both of them increased in breviscapine group. Conclusion： It is very important for controlling tumor metastasis and recurrence to recover host immunity after the tumor removal. Breviscapine can promote the restoration of host immunity, therefore it might he used for preventing metastasis and recurrence after breast cancer removal.