摘要
目的:探讨测定穿心莲内酯滴丸中穿心莲内酯含量的方法。方法:采用HPLC法测定,使用Hypersil—CN(150mm×4.6mm,5μm)色谱柱,以甲醇:水(40:60)为流动相,流速1.0ml/min,检测波长254nm。结果:穿心莲内酯在20.0~100.0gg/ml范围内有很好的线性关系,其线性方程为A—11484C+35642(n=5.r=0.9998),回收率为100.53%,RSD=0.39%(n=6)。结论:HPLC法测定穿心莲内酯滴丸中穿心莲内酯线性关系好、准确度高、稳定性好、专属性强,可作为该产品的定量控制方法。
Objective: To establish the determination method for the of andrographolide in andrographolide pill with HPLC. Methods: The HPI.C method was used at a Hypersil-- CN column ( 150mm ×4.6ram, 5μm) as analytical column, with the mobile phase of methanol--water(40:60). The flow rate was 1.0ml/min,and the detection wave-- length was 254nm. Results: The concentration of andrographolide was linearly related to its peak area in the rang of 20.0-100.0 μg/ml, the linear regression equation was A=11484C+35642 (n=5, r=0. 9998 ) . The recovery was 100.53% and RSD of 0.39 % (n= 63. Conclusion: The determination of andrographolide in andrographolide pill with HPLC has a good linear relation with the high accurate rate and the good stability, so it can be used for the quantitative analysis.
出处
《河南大学学报(医学版)》
CAS
2009年第3期200-201,231,共3页
Journal of Henan University:Medical Science
关键词
HPLC法
滴丸
穿心莲内酯
HPLC
Andrographolide pill
Andrographolide