摘要
目的：应用HPLC-ELSD法测定银杏叶胶囊中银杏内酯A、B、C及白果内酯的含量。方法：采用C18色谱柱（Kromasil,4.6×250mm,5μm）,以甲醇-水为流动相梯度洗脱,ELSD雾化温度为50℃,载气N2流速为1.2L/Min。结果：测得银杏内酯A、B、C及白果内酯分别在2.18μg-16.35μg、2.10μg-15.60μg、0.59μg-8.85μg、0.73μg-9.90μg线性关系良好;银杏内酯A、B、C及白果内酯的平均回收率分别为97.28%、97.68%、98.34%、99.52%。结论：该方法简单、准确可靠,分离效果好;本方法可用于银杏叶胶囊的质量评价。
Objective： To establish the quantitative method of Terpene Lactones in Ginkgo Capsule by HPLC--ELSD. Method.. The column used was a Diamonsil C18 （4.6 ×250mm）paked with a 5μm stationary phase . The mobile phase consisted of MeOH- H2O. The temperature of ELSD was 500C and the nebulizer nitrogen flow rate was 1.2L/Min. Result：The liner range of the four components was 2.18μg--16.3μg ,2, 10μg--15.60μg, 0.59μg--8.85μg,0. 73μg--9.90μg respectively. The mean recoveries of the four components were97.28%,97.68%,98.34%,99.52 % respectively. Conclusion： It was proved that the method with HPLC --ELSD was reliable,simple,well separated and precise ,which could be used as principle of quantitative analysis.
出处
《内蒙古中医药》
2009年第7期11-14,共4页
Inner Mongolia Journal of Traditional Chinese Medicine
