添加营养发芽诱导因子和抑制因子对压力诱导芽孢发芽的影响的研究

Study on the effect of pressure to spores germination by inducible factor and inhibitor

选择4种组合的营养发芽诱导因子和两组抑制因子来考察压力对芽孢发芽的影响,施压条件为0.1MPa/50℃,0.1MPa/80℃,500MPa/50℃,500MPa/80℃。添加的营养发芽诱导因子和抑制因子在常压下对2种菌的诱导现象和抑制现象都不明现。在加压的条件下,L-丙氨酸((50+10)mmol/L)在较低温度是对2种细菌芽孢都有营养诱导发芽现象,对凝结芽孢杆菌的诱导现象尤为明显,处理条件为500MPa、初温50℃时,凝结芽孢杆菌芽孢的发芽率约为5个数量级;4种营养组合的发芽诱导因素(L-丙氨酸+天门冬酰胺+果糖+葡萄糖)在温度较高时对发芽有明显的诱导作用,凝结芽孢杆菌的诱导发芽率为5.5个数量级,对嗜热脂肪芽孢杆菌的诱导发芽率为4.98。对于抑制因素,氯化镧的抑制作用十分明显,而TAME则只对凝结芽孢杆菌芽孢的发芽有抑制的作用,对嗜热脂肪芽孢杆菌芽孢抑制作用不明显。

Select four assemblage of nutritional induced factors and two assemblage of nutritional inhibitor to study the effect of pressure on the germination of spores. The pressure conditions is 0.1 MPa/50 ℃, 0.1 MPa/ 80 ℃, 500 MPa/50 ℃, 500 MPa/80 ℃. Effect of the added nutritional induced factors and inhibitor at the atmospheric Pressure to spores is not significant. In the pressurized conditions, L-alanine（（50＋10）mmol/L） at a relatively low temperature for two types of bacterial spores have germinated nutrition-induced phenomenon. The nutrition-induced phenomenon to Bacillus Coagulans is more significant. Under the condition of 500 MPa/50 ℃the germination rate of Bacillus cereus is about 5 magnitude. Four kinds of combinations of nutritional induced factors（L-alanine＋asparaginase＋fructose＋glucose） has significant induced effect. The germination rate of Bacillus cereus is 5.5 magnitude while the germination rate of Bacillus stearothermophilus is 4.98 magnitude. To the nutritional inhibitor, lanthanum chloride had obvious inhibition to two kinds spores while TAME only had inhibition to Bacillus coagulans and had no significant inhibition to Bacillus stearothermophilus.