三聚氰胺在蛋鸡体内和鸡蛋中的分布及代谢研究

Study on the distribution and metabolism of melamine in laying-hens and eggs

研究三聚氰胺在蛋鸡不同组织和鸡蛋不同部位中的分布和代谢规律。以蛋鸡为实验模型,采用超高效液相-串联质谱方法,检测在喂养含三聚氰胺的饲料后的不同时间、不同的鸡肌肉组织(鸡腿肉、鸡胸肉、血浆、鸡肾、鸡肝)及鸡蛋不同部位中三聚氰胺残留量。结果表明:三聚氰胺在蛋鸡不同组织和鸡蛋不同部位中的残留量有很大差异;给药期间,残留浓度大小的顺序为蛋清>肾脏>血浆>肝脏>鸡腿肉、鸡胸肉>鸡蛋黄;停药14d内,残留浓度大小的顺序为蛋黄>蛋清>血浆、肝脏、肾脏、肌肉组织。鸡蛋清、蛋黄消除速度低于鸡血浆、肝脏、肾脏、肌肉等组织,但到停药的21d时,三聚氰胺的残留量都下降到0.30mg/kg以下。

In this paper, the distribution and metabolism of melamine in the eggs of different organizations and different parts of laying-hens were studied. It was performed in laying hens, after feeding on containing feedstuff, UPLC-MSMS was used to detect melamine residues in different tissues of laying-hens and eggs at different times. The results showed that melamine in different parts of laying-hens and eggs are very different： in delivery period, the order of melamine residual concentration in different parts was： egg albumen〉kidney〉 plasma〉liver〉drumstick, breast〉yolk; at 14d after withdrawing melamine, the order of melamine residual concentration was： yolk〉egg albumen〉plasma, liver, kidney and muscle tissue. Melamine metabolized in egg albumen and yolk were slower than plasma, liver, kidney and muscle tissue. However, at 21 d after withdrawing, the melamine residues in all parts are reduced to below 0.30 mg/kg.