摘要
研究大豆异黄酮的体外抗脂质过氧化作用。用T-AOC方法测定在化学模拟体系中的总抗氧化能力,用八木变法测定过氧化脂质(LOOH)含量,用硫代巴比妥酸(TBA)法测定丙二醛(MDA)含量,用分光光度法测定肝线粒体膨胀程度。结果表明,大豆异黄酮体外总抗氧化能力较强,可抑制血浆及肝组织脂质过氧化,减轻肝线粒体膨胀程度,提示大豆异黄酮具有抗脂质过氧化作用。
The anti-lipid peroxidative effects of soybean isoflavones were investigated in vitro. The total antioxidant activity in modified chemical system was detected by T-AOC method, the content of LOOH（lipid hydroperoxide） was measured by Yagi＇s method, the content of MDA（malonyldialdehide） was measured by TBA （thiobarbituric acid） assay, the swelling of liver mitochondria were detected by spectrophotometric method. The result showed that soybean isoflavones exerted the high total antioxidative activities in vitro, inhibited the lipid peroxidation in plasma and liver, and suppressed the swelling of mitochondria. It is suggested that soybean isoflavones have the anti-lipid peroxidative effects.
出处
《食品科技》
CAS
北大核心
2009年第9期157-159,共3页
Food Science and Technology
基金
吉林省科技发展计划资助项目(200705428)
关键词
大豆
异黄酮
体外
脂质过氧化
soybean
isoflavones
in vitro
lipid peroxidation
