摘要
目的:比较蒽酮-硫酸法与苯酚-硫酸法测定枸杞子中多糖含量的差异。方法:分别采用上述两种方法利用紫外-可见分光光度计测定枸杞子中多糖的含量。结果:蒽酮-硫酸法测定结果高于苯酚-硫酸法,其差异有统计学意义。结论:苯酚-硫酸法测定结果更为准确合理,可考虑作为枸杞子中多糖含量测定的首选方法。
Objective: To compare difference in determination content between phenol-vitriolic colorimetry method and anthrone sulphuric acid colrimetry method for polysaccharides in Lycium barbarum. Methods: Above two methods were employed to determine content of polysaccharides by UV spectrophotography. Results: The determination value of anthrone-sulphuric acid colorimetry method is high than the phenol-vitriolic colorimetry method, and its values dissimilarity have statistical significance between two methods.Conclusion: The result of phenol-vitriolic colorimetry method is more reliable and accurate, it can be considered as the optimum assay method for polysaccharides in Lycium barbarum.
出处
《食品科技》
CAS
北大核心
2009年第9期270-272,共3页
Food Science and Technology
关键词
枸杞多糖
蒽酮硫酸法
苯酚硫酸法
含量测定
Lycium barbarum polysaccharide
anthrone sulphuric acid colorimetry
phenol-vitriolic colorimetry
content determination