蒽酮硫酸法与苯酚硫酸法测定枸杞子中多糖含量的比较

Comparison of methods in determination of polysaccharide in Lycium barbarum L.

目的:比较蒽酮-硫酸法与苯酚-硫酸法测定枸杞子中多糖含量的差异。方法:分别采用上述两种方法利用紫外-可见分光光度计测定枸杞子中多糖的含量。结果:蒽酮-硫酸法测定结果高于苯酚-硫酸法,其差异有统计学意义。结论:苯酚-硫酸法测定结果更为准确合理,可考虑作为枸杞子中多糖含量测定的首选方法。

Objective： To compare difference in determination content between phenol-vitriolic colorimetry method and anthrone sulphuric acid colrimetry method for polysaccharides in Lycium barbarum. Methods： Above two methods were employed to determine content of polysaccharides by UV spectrophotography. Results： The determination value of anthrone-sulphuric acid colorimetry method is high than the phenol-vitriolic colorimetry method, and its values dissimilarity have statistical significance between two methods.Conclusion： The result of phenol-vitriolic colorimetry method is more reliable and accurate, it can be considered as the optimum assay method for polysaccharides in Lycium barbarum.