摘要
从宁夏枸杞子提取得到的糖缀合物LBP经DEAEcelulose柱色谱得到5个组分,其中Lbp3,Lbp4和Lbp5分别经CMSephadexC50,SephadexG100,SephadexG50柱色谱得到均一组分LbGp3,LbGp4和LbGp5。N含量:LbGp3083%,LbGp4172%,LbGp5958%。总糖含量:LbGp3936%,LbGp4856%,LbGp586%。分子量:LbGp3925×104,LbGp42148×104,LbGp5237×104。糖组成为:LbGp3:Ara∶Glc=1∶1,LbGp4:Ara∶Gal∶Rha∶Glc=15∶25∶043∶023,LbGp5:Rha∶Ara∶Xyl∶Gal∶Man∶Glc=033∶052∶042∶094∶085∶1。初步分析表明LbGp4是O连接的糖蛋白。
Three glycoconjugates, LbGp3, LbGp4 and LbGp5, were isolated from the fruit of Lycium barbarum L. Molecular weights of LbGp3, LbGp4 and LbGp5 were 9.25×10~4, 21.48×10~4 and 2.37×10~4, respectively. Carbohydrate contents of LbGp3, LbGP4 and LbGp5 were 93.6%, 85.6%, 8.6%, respectivly. LbGp3 was composed of Ara and Gal in a molar ratio of 1∶1. LbGp4 was composed of Ara, Gal, Rha and Glc in a molar ratio of 1.5∶2.5∶0.43∶0.23. LbGp5 was composed of Rha, Ara, Xyl, Gal, Man and Glc in a molar ratio of 0.33∶0.52∶0.42∶0.94∶0.85∶1. Elemental analysis of N contents: LbGp3 0.83%, LbGp4 1.72%, LbGp5 9.58%. The linkage between the glycan and protein may be of Olinkage in LbGp4.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第7期512-516,共5页
Acta Pharmaceutica Sinica