神经鞘氨醇磷酸胆碱对原代培养人角质形成细胞DNA合成影响

Effect of DNA synthesis of primary cultures of human keratinocyte by sphingosylphorylcholine

目的观察神经鞘氨醇磷酸胆碱(Sphingosylphosphorylcholine,SPC)对原代培养的人角质形成细胞DNA合成影响,并选择最适浓度。方法原代培养人角质形成细胞,加入不同浓度的SPC,之后测定3H-TdR掺入量。结果SPC的深度为2μM时,3H-TdR掺入量最高,当SPC的浓度超过2μM时3H-TdR掺入量有下降趋势,但SPC的浓度5μM时3H-TdR掺入量仍然很高。SPC的浓度为20μM时3H-TdR掺入量降到最低点。结论SPC浓度为2μM时促进原代培养的人角质形成细胞DNA合成能力最强,但浓度超过20μM时显示有细胞毒性。SPC在促进创伤愈合的治疗中将有很好的应用前景。

Objective To investigate the influence of Sphingosylphosphoryleholine（SPC）on the DNA synthesis of primary culture of human keratinoeyte. Methods Quantify the ineorporation of [^3H] thymidine among primary cultme of human keratinoeyte after treated by dose-dependent SPC. Results The DNA [ ^3H] incorporation reaches its maximun at SPC eoneentratiun of 5 μM and falls into the lowest at SPC concentration of 10 μM. Conclusion SPC can play an irnprotant role in stimulating the DNA synthesis of primary euhures of human keratinoeyte within the concentrate of 2 to 10 μM. But SPC will also show eytotoxie effect if it concentration beyond 20 μ. SPC may show fabulous foreground in the treatment of wound healing.