摘要
目的比较不同血小板功能检测方法对阿司匹林疗效判断及其临床应用价值。方法采用血小板功能分析仪-100(platelet function analyzer,PFA-100)和光学法,应用不同的诱导剂(花生四烯酸、二磷腺苷酸、肾上腺素和胶原)测定血小板聚集率,分别对40例正常人、51例原发性高血压未服用阿司匹林患者及54例原发性高血压服用阿司匹林(100 mg/d)患者进行血小板功能测定,并以P-选择素水平的检测结果为对照,分析2种方法检测结果。结果原发性高血压服用阿司匹林组(服药组)中4种诱导剂测得的光学法血小板聚集率较正常组和临床对照组均明显下降(P均<0.05);服药组中PFA-100 CEPI测得的闭合时间(CT)值较正常组和临床对照组显著延长(P<0.01)。光学法血小板聚集检测的AR组及PFA-100测得的PFA-AR组中P-选择素的水平均显著增高。PFA-100对AR检出的敏感度较光学法血小板聚集高。PFA-100 CEPI检测盒与临床事件的一致性较光学法血小板聚集高。结论光学法血小板聚集检测阿司匹林疗效,以AA作为诱导剂更敏感、特异;使用不同诱导剂进行监测有助于对更全面了解阿司匹林疗效;PFA-100较传统光学法血小板聚集有更好的敏感性,与临床事件的一致性高。
Objective To measure the clinical effieacy of aspirin between different test of platelet function. Methods To measure the platelet function by using PFA-100 and optical aggregonletry by four reducer among 40 normal persons, 51 primary hypertension patients without taking aspirin and 54 primary hypertension patients taking aspirin 100 mg/d and analyse the agreemence between two methods by contrast with P-seleetin level. Results The platelet aggregometry reduced by AA, ADP, EPI and COL were significandy decreased than the normal group and clinical control group（ P 〈 0.05） ;PFA-100 CEPI CTs in medication group were significandy longer than the normal group and clinical control group（ P 〈 0. 01 ）. The P-seletin level of AR detecting by two methods were both significant higher. The sensitivity of PFA-100 is higher than optical platelet aggregometry. The consistency between PFA-100 CEPI cartridge vs clinical events was higher than that between optical platelet aggregometry vs clinical events. Conclusion AA is the most sensitive reducer in optical platelet function measuring aspirin effcity. PFA-100 is another both sensitive and convenient method in measuring the platelet function in aspirin-taking persons.
出处
《中国实验诊断学》
北大核心
2009年第9期1179-1182,共4页
Chinese Journal of Laboratory Diagnosis
基金
上海市科委基金(074119601)
