ELISA检测592例乙肝表面抗原复检结果的分析

Anlysis of 592 HBsAg review results manually dectected by ELISA

目的总结分析592例ELISA手工检测乙肝表面抗原的复检结果特点,进一步提高ELISA手工检测的质量。方法以0.7≤S/CO<10作为复检范围,从67 070例ELISA手工检测HBsAg的结果中筛选出592例进行复检,两次结果一致则报告结果;如不一致则再次ELISA复检或采用MEIA进行复检,以两次一致的ELISA或MEIA结果报告。分析初检时造成假阳性或假阴性结果的原因。结果按照2007年上海市临床检验中心复检要求,以0.7≤S/CO<2为复检范围,复检率为0.48%(325/67 070);本实验室扩大复检范围,以0.7≤S/CO<10为复检范围,复检率为0.88%(592/67 070),初复检结果符合率为65.88%(390/592),初检假阴性率为1.35%(8/592),初检假阳性率为32.77%(194/592)。结论严格遵守操作规程,根据实验室实际情况扩大复检范围,确保实验结果准确性。

Objective To anlysis 592 HBsAg review results manually detected by ELISA and improve the quality of ELISA operation. Methods As 0.7 ≤ S/CO 〈 10 was the review scope, 592 samples were selected to review from 67070 samples manually detected by ELISA, then two consistent results were reported;If the results were not consistent, the sample detected again by ELISA or MEIA and reported the same results, the reasons of fasle positive or fasle negative results were analyzed. Results According to the review requirements of shanghai clinical laboratory centre in 2007, the review rate was 0.48 % （325/67070） using 0.7 ≤ S/CO 〈 2 as the review scope; while the rate was 0. 88 % （592/67070） using 0.7 ≤ S/CO 〈 10 as the expanding review scope in our laboratory. The coincidence of two results was 65.88 % （390/592）, but the fasle negative rate and the fasle positive rate were 1.35 % （ 8/592 ）, 32.77 % （194/592）respectively. Conclusion Strictly abiding by the operating procedures, and expanding the review scope according to actual situation of laboratory, is to ensure accuracy of experimental results.