薄层扫描法测定黄芪生脉颗粒中黄芪甲甙含量

Determination of astragaloside Ⅳ in Huangqi Shengmai Granules by TLCS

目的:制订黄芪生脉颗粒中黄芪甲甙含量测定方法。方法:双波长薄层扫描法,经乙醚洗涤、正丁醇提取和D101大孔吸附树脂柱层析法制备样品,以氯仿-甲醇-水(13:7:2)下层液为展开剂,检测波长为510nm,参比波长为700nm。结果:加标回收率平均为98.7％(RSD=2.0％,n=6),标准曲线r=0.9966,重复性RSD=1.4％(n=5),精密度RSD=2.0％(n=6)。结论:方法稳定、可靠,可作为该制剂的质量控制方法之一。

OBJECTIVE:To develop the method for content determination of astragaloside IV in Huangqi Shengmai Granules.METHODS:Using Dual - wavelength TLC - scanning,the sample was prepared through the column full of D101 macroporous resin with n - butanol extract from the aque ous solution of the granules which had been washed by ether, developer is chloroform - methanol - water(13:10:7)(stored at less than 10℃ ,the lower) .detecter is 510nm,reference is 700nm.RESULTS:The average recovery is 98.7%(RSD = 2.0%,n = 6),with good linear relationship(r = 0.9966) and reproducibitity(RSD = 1.4%, n = 5) and precision(RSD = 2.0%, n = 6). CONCLUSION:The method is accurate, reliabe, can be one of method for quality control of the granules.