摘要
目的了解受试样品三唑磷原药(92%)的亚慢性经口毒性效应,取得该样品亚慢性经口的最大无作用剂量参数,为安全生产及慢性毒性实验提供剂量参考依据。方法依据GB 15670-1995《农药登记毒理学试验方法》对其进行为期90 d的毒性实验。结果在整个染毒期间,高、中剂量组雌性大鼠的天冬氨酸氨基转移酶(AST)明显高于对照组(P<0.05),高剂量组雌性大鼠的碱性磷酸酶(ALP)明显低于对照组(P<0.05);从染毒第2周开始至13周试验结束时,高剂量组雄性大鼠及高、中剂量组雌性大鼠胆碱酯酶活力(CHE)受到抑制,明显低于对照组(P<0.05或P<0.01),且存在剂量-反应关系。病理组织学检查高剂量组雄、雌性部分动物肝脏浊肿,胞质疏松,汇管区少许炎细胞。结论在该试验条件下,三唑磷原药(92%)对大鼠亚慢性经口毒性最大无作用剂量为雄性为0.10 mg/(kg.d),雌性为0.07 mg/(kg.d)。CHE可作为该农药敏感的生物标志物。
[Objective] To identify the performance of subchronic oral toxicity of raw Triazophos {92% } samples, to obtain the maximum non-effect dose, and provide dose reference for safe production and chronic toxicity test. [ Methods ] 90-day-toxicity-test was performed based on GB 15670 - 1995 ＂Toxicological Test Method for Pesticide Registration＂. [ Results] During the entire expo- sure, AST of female rats in high, medium dose groups were significantly higher than that of control group { P 〈 0.05 } , ALP of female rats in high dose group was significantly lower than that of control group{ P 〈 0.05} ; from the 2nd week of exposure to the 13th week of test end, CHE of male rats in high dose group and female rats in high, medium dose groups were inhibited, and were significandy lower than control group { P 〈0. 05 or P 〈0 . 01 } , with dose-response relationship. Cloudy swelling of liver, cytoplasms loose and inflammatory ceils in portal area were detected by using histopathologieal examination. [ Conclusion] Under the experimental conditions, the maximum non-effect dose of raw Triazophos {92% } is 0.10 mg./（ kg · d） on male rats and O. 07 mg/（ kg · d} on female rats. CHE can be used as the sensitive biomarker of this pesticide.
出处
《职业与健康》
CAS
2009年第19期2025-2028,共4页
Occupation and Health
