超高静压协同热处理对2种细菌芽孢内容物的影响

Study the Effects of Hot-press Collaborative on the Contents of Two Bacteria Bacillus Spores

选用凝结芽孢杆菌和嗜热脂肪芽孢杆菌作为研究对象,采用紫外分光光度法和比色法测定热压协同处理后2种细菌芽孢内容物(蛋白质、核酸、吡啶-2,6-二羧酸)的泄漏量;扫描电镜和透射电镜观察热压协同处理后2种细菌芽孢内外部结构变化。结果表明:透射电镜观察结果、DPA的泄漏量和灭活率3者之间在相同的热压处理条件下显示的规律一致,可以相互佐证,互为解释,从其中一个方面变化规律可以反映到另外2个方面的变化。

Selected Bacillus coagulans and Bacillus stearothermophilus as the study objects and used ultraviolet speetrophotometry and colorimetrie determination to detect the quantity of two bacteria＇s inclusions after processing with hot coordinating press. The contents include protein, nucleic acid and pyridine 2, 6-dicarboxylic acid. This study also observed the change of internal and external structure by scanning electron microscopy and transmission electron microscopy. Results showed that pattern of the transmission electron microscopy observations, leakage of pyridine 2, 6-dicarboxylic acid and inactivation rate during the hot coordinating press is consistent with each other, and the leakage of pyridine 2, 6- dicarboxylic acid reflected the changes in mortality and transmission electron microscopy observations.