摘要
目的探讨2种方法联合检测对提高输血安全性的价值。方法采用酶联免疫吸附测定(ELISA)和荧光定量PCR技术,分别对确诊或疑似乙型肝炎血液标本(346份)和初筛合格献血员标本(300份)进行HBV血清标志物和DNA含量检测。结果经ELISA法检测,346份确诊或疑似乙型肝炎血液标本,HBV标志物阴性62份,其中检出HBV-DNA阳性1份(1.5%);在300份初筛合格的献血员标本中,检出HBV-DNA阳性6份(2.0%),其中222份ELISA法全阴性的标本,检出HBV-DNA阳性2份(0.9%)。结论在HBsAg阴性者中,也可能存在HBV感染;HBV血清标志物和DNA的联合检测,对提高输血安全性有重要意义。
[ Objective] To discuss the value of combined detection in improving blood transfusion safety. [ Methods ] HBV serum markers and DNA contents in blood samples from confirmed or suspected HBV carriers 1346 samples ） and primary screening qualified blood donors （300 samples） were detected with ELISA and fluorescence quantitative PCR. [ Results] Among 346 serum samples from confirmed or suspected HBV carriers detected by ELISA, 62 cases were HBV serum markers negative, in which 1 case was detected the HBV-DNA positive （ 1.5% ） ; among 300 samples of primary screening qualified blood donor serum, 6 cases of HBV-DNA were positive （2.0%）. Among 222 negative samples detected by ELISA, 2 cases were HBV-DNA positive （0.9% ）. [ Conclusion] HBV infection may exist in the HBsAg negative people ; combined detection of HBV serum markers and DNA contents plays a significant role in improving the safety of blood transfusion.
出处
《职业与健康》
CAS
2009年第20期2166-2168,共3页
Occupation and Health
