DAZ及DAZH基因与精子发生的相关性分析

Analysis of the DAZ and DAZH genes in spermatogenesis

ＤＡＺ基因（ｄｅｌｅｔｅｄｉｎａｚｏｏｓｐｅｒｍｉａ）是无精子因子（ＡＺＦ）的重要候选成分，它的缺失可致无精子或严重少精子症。人第３号染色体上存在着ＤＡＺ的同源基因ＤＡＺＨ（ＤＡＺｈｏｍｏｌｏｇｕｅ），为探讨ＤＡＺ和ＤＡＺＨ基因在精子发生中的作用，我们用聚合酶链反应（ＰＣＲ）方法检测了６６名正常生育男性和９０例原发性不育男性基因组ＤＮＡ的ＤＡＺ、ＤＡＺＨ基因。结果：原发性不育男性中１２例ＤＡＺ缺失（无精子症８例，严重少精子症４例）；正常男性ＤＡＺ无一缺失；在正常男性和不育男性中均有ＤＡＺＨ缺失。结果进一步证实ＤＡＺ为ＡＺＦ的候选成分，而ＤＡＺＨ基因与精子发生似无直接的相关性。

おbjective:To investigate the role of the DAZ and DAZH genes in spermatogenesis.Design:An experimental study.Subjects: Sixty six normal men and 90 men with azoospermia or severe oligozoospermia.Methods: Polymerase chain reaction.Results:Twelve of the 90 patients had DAZ deletion(8 azoospermic and 4 oligozoospermic),while no DAZ deletion was detected in any of the 66 normal men.DAZH deletion was found in both normal men and idiopathic infertile men.Conclusion:These data provide further evidence that DAZ is a candidate for AZF,and it seems that there is no relativity between the DAZH gene and spermatogenesis.