摘要
从新疆伊犁农牧民传统发酵的酸马奶中分离筛选到呈革兰氏染色阳性,秆状,编号为H-1的菌株。通过对此菌株进行形态观察和普通生理分析,并测定该菌株降解胆固醇的活力,通过16S r DNA序列分析表明,分离菌株H-1与Lactobacillus casei具有100%相似性,说明此菌株即为干酪乳杆菌。使用ClustalX1.8程序和Mega2程序将该菌株与报道的相关乳酸菌进行系统发育分析,揭示了16S r DNA序列分析在菌株鉴定的正确性。
Bacterial strain baeuliform H- 1 Gram- positive was isolated from traditional fermenting koumiss in Ili,Xinjiang. The activity of bacterial strain eholesterol degradation was determined. The analysis on 165 rDNA sequenee indicated that the isolated bacteria strain H- 1 was similar to Laetobaeillus easei by 100%. The phylogness analysis on the bacteria strain and the reported related laetie acid bacteria, which revealed that the identification of sequence analysis on 165 rDNA was correct.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2009年第5期1121-1125,共5页
Xinjiang Agricultural Sciences
基金
国家科技部国际科技合作重点项目"乳用马品种引进及杂交改良初步试验研究"(2005DFA30760)
新疆维吾尔自治区科技厅重点攻关项目"引进乳用马品种开展杂交改良试验研究"(200541101)
益生菌定向筛选与功能开发关键技术研究(2007AA10Z357)
国家高技术研究发展计划(863计划)
关键词
乳酸菌
胆固醇降解
16S
r
DNA序列
系统发育
lactic acid bacteria
cholesterol degradation
16S rDNA sequence
phylogenetie analysis
