兔肝细胞的脾脏移植和定位初步研究

Study of Transplantation and Localization of Rabbit Hepatocyte in Spleen

【目的】探讨肝细胞体内移植的途径和定位分析。【方法】从新西兰白兔的肝脏中分离获得原代肝细胞，经纯化培养后经脾动脉插管或注射移植到脾脏，在不同时间注射肝细胞特异性增强剂（扎贝葡胺），观察移植后脾脏核磁信号的变化和移植肝细胞的存活，并分析细胞移植前后肝功能变化。【结果】肝细胞移植入脾脏一段时间后受损肝功能明显改善。脾脏内可见肝细胞信号，d，信号最强，以后逐渐衰退并持续14d以上。【结论】肝细胞脾内移植是可行的；应用肝细胞增强剂进行MRl分析可成为肝细胞移植无创伤检测手段。

[Objective] To investigate the transplantation and localization of hepatocyte in spleen. [Methods]The primary rabbit hepatocytes were isolated from normal liver tissue of new Zealand rabbits. After cul- ture, hepatocytes were transplanted into the spleen of rabbit with liver failure. The changes of liver function and MRI signals of the spleen were analyzed after gadobenate dimeglumine was injected. [Results] The symp- toms were improved. Serum levels of ALT and NH3 were significantly reduced after hepatocyte transplanta- tion. Signals of hepatocytes were observed in the spleen. [Conclusion]Hepatocyte transplantation is availabili- ty in spleen. Contrast enhanced MRI can be a new method for localizable analysis of transplanted hepatocytes.