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兔肝细胞的脾脏移植和定位初步研究 被引量:3

Study of Transplantation and Localization of Rabbit Hepatocyte in Spleen
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摘要 【目的】探讨肝细胞体内移植的途径和定位分析。【方法】从新西兰白兔的肝脏中分离获得原代肝细胞,经纯化培养后经脾动脉插管或注射移植到脾脏,在不同时间注射肝细胞特异性增强剂(扎贝葡胺),观察移植后脾脏核磁信号的变化和移植肝细胞的存活,并分析细胞移植前后肝功能变化。【结果】肝细胞移植入脾脏一段时间后受损肝功能明显改善。脾脏内可见肝细胞信号,d,信号最强,以后逐渐衰退并持续14d以上。【结论】肝细胞脾内移植是可行的;应用肝细胞增强剂进行MRl分析可成为肝细胞移植无创伤检测手段。 [Objective] To investigate the transplantation and localization of hepatocyte in spleen. [Methods]The primary rabbit hepatocytes were isolated from normal liver tissue of new Zealand rabbits. After cul- ture, hepatocytes were transplanted into the spleen of rabbit with liver failure. The changes of liver function and MRI signals of the spleen were analyzed after gadobenate dimeglumine was injected. [Results] The symp- toms were improved. Serum levels of ALT and NH3 were significantly reduced after hepatocyte transplanta- tion. Signals of hepatocytes were observed in the spleen. [Conclusion]Hepatocyte transplantation is availabili- ty in spleen. Contrast enhanced MRI can be a new method for localizable analysis of transplanted hepatocytes.
出处 《医学临床研究》 CAS 2009年第9期1569-1571,1576,共4页 Journal of Clinical Research
基金 [基金项目]本课题由湖南省科技厅重点项目(2008SK2004)、湖南省卫生厅科研基金(B2007135)和湖南省人民医院仁术基金资助
关键词 脾/移植 肝/细胞学 spleen/TR rabbits liver/CY
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参考文献20

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二级参考文献26

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