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TROP2基因mRNA在胃癌组织中的表达及其意义

Expression of TROP2 mRNA in Gastric Cancer and Its Significance
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摘要 【目的】探讨TROP2基因mRNA表达在胃癌发病过程的作用。【方法】Trizol方法常规提取42例放有RNAlater在液氮保存的新鲜胃癌和对应的癌旁组织总RNA。利用DnaseI将残余的DNA去除后,将这些总RNA反转录成cDNA后,利用实时荧光定量PCR的方法检测TROP2基因在两种组织中的表达情况,分析其与胃癌转移相关的临床病理参数之间的关系。【结果】与癌旁胃黏膜组织相比,TROP2基因在胃癌组织中呈明显高表达。此外,在有转移的癌组织中,TROP2基因表达比非转移的癌组织明显增强。【结论】TROP2基因高表达提示其参与了胃癌的发病过程,并可能与胃癌转移发生相关。 [Objective] To explore the expression of TROP'2 encoding a transmembrane calcium signal transducer which is involved in the regulation of cell-cell adhesion and considered as an oncogenic factor that is closely associated with the pathogenesis of tumors, and to study its role in the pathogenesis of gastric cancer. [Methods] Total RNA of 42 fresh gastric cancer samples and their correspoding peritumor samples were extracted by routine Trizol way. After trace DNA was digested, total RNA was transcripted into cDNA, and the expression of TROP2 gene was detected in these samples by reaLtime PCR. Finally, the correlation between TROP2 expression and clinical pathological feature of metas- tasis was analyzed in gastric cancer. [ResuhslComparing to the peritumor samples, the expression of TROP2 was markedly increased in gastric cancer samples. In addition, TROP2 expression in gastric cancer samples with metastasis characteristics was obvious higher than that in non-metastatic gastric cancer samples. [ConclusionlThe high expression of TROP2 hinted that TROP2 may promote the pathogenesis of gastric cancer and is likely to be associated with the me tastasis of gastric cancer.
出处 《医学临床研究》 CAS 2009年第9期1690-1692,共3页 Journal of Clinical Research
关键词 胃肿瘤/病理学 基因 RNA 信使 stomach neoplasms/PA genes RNA,messenger
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参考文献6

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