二醇柱高效液相色谱法测定人参提取液中的人参皂苷

Determination of ginsenosides in ginseng fluidextract using high-performance liquid chromatography with diol column

采用二醇柱,在等度洗脱条件下,建立了高效液相色谱(HPLC)定量分析人参提取液中人参皂苷的检测方法.优化的色谱条件:LiChrospher100 Diol柱(250 mm x2 mm i.d.,5μm),流动相为乙腈-水(体积比为82∶18),流速为1.0 L.min-1,检测波长为200 nm,柱温为15℃.研究结果表明:在较低温度下,采用二醇柱分析7种主要人参皂苷,具有分析速度快,不需要梯度洗脱程序,方法简单等优点.

A high-performance liquid chromatographic method was developed for determination of ginsenosides in ginseng fluidextract using diol eolumn with isocratic elution. The optimization conditions were carried out on a LiChrospher 100 diol column （250 inm × 2 mmi. d. ,5μm） with acetonitfile and water （82：18 v/v） as mo- bile phase at a flow rate of 1.0 mL/min. The detection wavelength was 200 nm, and the column temperature was 15℃. The results showed that the proposed method is rapid and simple for separation of seven ginsen- osides without gradient elution program at lower temperature.