来自莫迦小麦(T. macha)的T型恢复基因Rf3和K型不育基因rfv1的SSR标记分析

SSR Markers Analysis of T-type Restorer Gene Rf3 and K-type Male Sterile Gene rfv1 Derived from Triticum macha L.

为了建立非1B/1R类型K型小麦细胞质雄性不育系的分子标记辅助选育技术体系,对基础遗传材料Tm3314来自莫迦小麦1BS染色体的T型恢复基因Rf3和K型不育基因rfv1进行了分子标记定位。以Tm3314和T型不育系T504A的杂交F2代作为定位群体,利用分离集团分析法(BSA),从1BS染色体上10对SSR引物中筛选出与目的基因连锁的2个SSR标记Xbarc8和Xgwm18。然后结合(T504A/Tm3314)F2群体在T型细胞质下的育性分离情况和F2可育株与K型不育系K119A测交所得的K型细胞质下的育性结果,运用Mapmaker 3.0b软件进行连锁分析,结果表明,Xbarc8和Xgwm18与Rf3基因的遗传距离分别为5.5 cM和8.1 cM,与rfv1基因的遗传距离分别为22.2 cM和19.6 cM,且2个SSR标记位于两个育性基因之间。

In order to establish a marker-assisted selection system for breeding non-lB/lR K-type cytoplasm male sterile（K-CMS）wheat lines, the molecular markers of T-type restorer gene Rf3 and K-type male sterile gene rful in Tm3314 （the two fertility genes derived from tBS chromosome of Triticum macha） were studied. A F2 population derived from crossing between Tm3314 and T-type male sterile line T504A was used for molecular mapping of the two fertility genes. The sterile and fertile near isogenic bulks was constructed from above F2 population. By using Bulked Segregant Analysis （BSA） strategy, ten SSR primers on wheat lBS chromosome were screened, and two SSR markers （Xbarc 8 and Xgwm 18） were found to be linked to fertility genes. Combined with fertility phenotypes of F2 individuals under the background of T. timophvee cytoplasm and F2 fertile individuals under the background of Aegilops kotschyi cytoplasm （which deduced from test-crossing with K CMS line K119A）, linkage between the two SSR markers and the two fertility genes were analyzed with Mapmaker 3.0b software. The results showed that the two SSR markers （Xbarc8 and Xgwm18） linked to Rf3 gene with distances of 5.5 cM and 8.1 cM, re spectively, linked to rful gene with distances of 22.2 cM and 19.6 cM, respectively. Furthermore, the two SSR markers were located between the Rf3 gene and the rful gene.