圣愈汤对环磷酰胺诱导的免疫低下小鼠T细胞亚群和细胞因子的影响

Effect of Shengyu decoction on changing T cell subgroups and cytokine levels in cyclophamide-induced mice

目的观察圣愈汤对免疫低下小鼠T细胞亚群和细胞因子的影响。方法选用昆明种小鼠,环磷酰胺诱导建立免疫低下小鼠模型,用不同剂量的圣愈汤经灌胃给药,每天1次。用药第30天时,称小鼠体重,摘眼球取血,肝素抗凝,采用免疫荧光及流式细胞术分析外周血T细胞亚群的改变,ELISA法检测血浆中IL-2及IL-4的含量。结果各组小鼠外周血的CD8+T细胞的百分率差异无显著性(>0.05)。环磷酰胺组小鼠外周血CD4+T的百分率及CD4+/CD8+的比值比对照组低(<0.05)。环磷酰胺+低剂量组的CD4+T的百分率及CD4+/CD8+的比值与环磷酰胺组高。环磷酰胺+高剂量组与环磷酰胺+免煎组的CD3+T、CD4+T的百分率及CD4+/CD8+的比值比环磷酰胺组高。环磷酰胺组小鼠血浆中细胞因子IL-2及IL-4的浓度比对照组低(<0.05);环磷酰胺+低剂量组、环磷酰胺+高剂量组以及环磷酰胺+免煎组血浆中IL-2及IL-4浓度比环磷酰胺组高,具有统计学意义(<0.05)。环磷酰胺+高剂量组与环磷酰胺+低剂量组IL-2浓度差异也有显著性(<0.05),但与环磷酰胺+免煎组比较差异无显著性(0.05)。结论圣愈汤能纠正环磷酰胺所致的免疫低下小鼠T细胞亚群的异常,升高血液中IL-2及IL-4的含量,从而提高机体的免疫应答水平。

Objective To investigate the effects of Shengyu decoction （SYD） on T cell subgroups and cytokine levels in immuno deficiency mice. Methods Kunming type mice were injected with cyclophosphamide to establish immuno deficiency mice model. The immuno deficiency mice were fed with low dose, high dose SYD respectively,once a day. On the 30th day, the mice were weighted; blood and the plasma was separated to measure the cytokine levels by ELISA test, and the T cell subgroups of CD3＋, CD4＋and CD8＋ were measured by flow cytometor analysis system. Results Cyclophosphamide remarkedly resulted in decreasing of CD3＋T cell, CD4＋T cell, CD4＋/CDS＋ratio, IL-2 and IL-4 production. SYD could increase the percentage of CD3＋T cell, CD4＋T cell and CD4＋/CD8＋ ratio obviously in immuno deficiency mice compared to those in cyclophosphamide group （p〈0.05）. The plasma level of IL-2 and IL-4 of immune deficit mice could be significantly improved by SYD. Conclusion SYD can immunomodulate the unusual distribution caused by cyclophosphamide among T cell subgroups of the mice with low immunity, improve the percentage of CD3＋T cell, CD4＋T cell and the ratio of CD4＋/CDS＋in mice, and promote the secretion of IL-2, IL-4 cytokines in mice with low immunity.