急性白血病药敏试验与临床缓解的回顾性研究(英文)

The Retrospective Study on Drug Sensitive Test of Acute Leukemia and Clinical Remission

目的 为寻找快速,经济,简便,可靠的白血病药敏试验方法.方法 回顾MTT法检测的85例初治急性白血病体外药敏试验与临床实际缓解的关系.结果 总阳性符合率为70%,阴性符合率为80%,其中AML阳性符合率73%,阴性符合率70%,ALL阳性与阴性符合率分别为61%,和100%,AML阳性符合率高于ALL,但无统计学差异(P>0.05).AML和ALL组中白血病细胞对一种化疗药物敏感率分别为71%和86%.AML各亚型临床符合率为67%～100%,ALL各亚型为64%～100%,均无统计学差异(P>0.05).分析实验结果与临床实际不符的病例,药敏与CFU—L同步检测的16例中12例有白血病集落自发性生长.结论提示MTT药敏试验可以指导临床用药,是一项值得在基层医院推广的试验.

Objective To finda rapid, economical, simple and reliable method of drug sensitivity for acute leukemia. Methods The retrospective study on relationship between drug sensitivity with MTT method in vitro and clinical remission rates for 85 cases for the first treatment of acute leukemia was performed. Results Total positive coincidence rate was 70% and negative coincidence rate was 80%. The positive coincidence rate of AML was 70% and negative coincidence rate was 80%, The positive coincidence rate of AML was 73% and negative coincidence rate was 70%. respectively. Meanwhile the positive coincidence rate of ALL was 61% and negative coincidence rate was 100%, reapectively. The positive rate of AML was higher than that of ALL, but there was no statistical difference(P>0.05) The rate of cells of AML and ALL sensitized to one kind of chemotherapic drug was 71% and 86%, respectiveiy. The positive coincidence rates of subtypes of AML were 67%-100%, and ones of subtypes of ALL were 64%-100%. Also, there was no statistical differece(P>0.05). The uncoincidental results of experimental report and clinical condition were analysed. According to experimental results of drug sensitive test and CPU-L assay simultaneously, 12 ones out of 16 cases showed independent colonies and clusters growth type. Conclusions The MTT drag sensitive test might be used directively for clinics. It should be an extensively used test for basic hospital.