慢性乙型肝炎患者血清HBV—DNA定量与定性检测的比较

The Comparison of Blot Hybridization Test and Quantitatitive PCR For Detection of HBV-DNA in the Patints with Chronic Hepatitis B

同时应用定量PCR法和斑点杂交法对155例HBsAg阳性的慢性乙型肝炎患者的血清HBVDNA进行检测.以探讨两者的相互关系.结果显示斑点杂交法检出66例阳性(47.6%),定量PCR法检出110例(71.0%).HBV-DNA于2.54拷贝出现斑点杂交法阳性,随着HBV—DNA拷贝数的增加,斑点杂交法阳性增强,表明两种方法的阳性呈正相关关系.定量PCR法比斑点杂交法灵敏且可定量,但试剂昂贵;而斑点杂交法简便易行,适合基层单位应用.故建议临床宜先用斑点杂交法检测HBV—DNA,再配合定量PCR法以提高检出率并进行HBV-DNA定量.

The aim of this paper is to compare the relationship between blot hybridization test and quantitative reverse transcriptional chain reaction (PCR) for the detection of HBV-DNA in 155 cases of patients with chronic hepatitis B. The results indicated that HBv-DNA was positive in 66 cases (47.6%) by blot hybridization test, while HBV-DNA was positive in 110 cases (71.0%) by quantitative PCR. Positive results was found by blot hybridization test in 102.54 copy of HBV-DNA. With the increase of HBV-DNA copy. the positive rate also increased by both blot hybridization test and quantitative PCR. This suggested that there was a strong correlation between two methods. Quantitative PCR was sensitive and could quantitate HBV-DNA, but expensive as compare with blot hybridization test. Blot hybridization test was imple and could be used in rural hospital. Therefor, we should firstly use blot hybridization test for the detection of HBV - DNA, then combined with quantitative PCR in order to enhance the positive rate and quantitate HBV-DNA.