实验性犬心肌梗塞和溶栓后再闭塞时血浆中GMP-140、TXB_2的变化

CHANGES OF PLASMA GMP-140 AND TXB_2 LEVELS DURING EXPERIMENTAL CORONARY THROMBOSIS AND REOCCLUSION AFTER RECANALIZATION IN DOGS

本文采用放免法测定实验性犬心肌梗塞和溶栓后再闭塞时血浆中GMP-140、TXB_2、6-K-PGFl_α的含量变化,并探讨其与心肌梗塞溶栓后再闭塞的关系。结果表明,在血栓形成时血浆中TXA_2稳定代谢产物TXB_2水平显著升高(p<0.05),在溶栓后再闭塞率高(87.5％)的非治疗组(B组),在溶栓后4小时至溶栓后3天期间呈进行性升高(p<0.01),而在再闭塞率低(16.7％)的API0134治疗组(A组)溶栓后4小时即较血栓形成时明显下降,且呈逐渐下降趋势(p<0.01),3天后已接近正常水平,而血浆中GMP-140水平在血栓形成时,溶栓后4小时～3天期间均明显升高(p<0.05),B组在30天时血浆中GMP-140仍升高(p<0.05),而A组在用API0134 30天时血浆中GMP-140已接近正常水平,血浆中6-K-PGFl_α水平在A、B两组之间及各不同时间均无明显差异。提示血浆GMP-140水平与TXB_2在血栓形成和再闭塞过程中变化趋势相似,动态测定GMP-140和TXB_2对于溶栓后再闭塞的发生可能有一定的预报价值,同时也可能为临床上判断溶栓后再闭塞提供一项新的诊断指标。

Dynamic changes of plasma platelet membrane glucoprotein-140 (GMP-140) and TXB2 levels were measured with RIA during experimental coronary thrombosis and reocclusion after thrombolytic therapy in dogs. The results showed that in group B dogs (nontreated with platalet inhibitor) with high reocclusion rate (87.5), plasma TXB2 and GMP-140 levels were elevated during thrombosis and remained high within thirty days after thrombolytic therapy with urokinase. In group A (platelet inhibitor API0134 treated) with much lower reocclusion rate(16.7%), plasma TXB2 and GMP-140 also increased at the time of thrombosis but at 4 hours, three days and thirty days after thromblytic therapy these two parameters were significantly lower than those in group B. It was shown that plasma TXB2 and GMP-140 levels changed parellelty during thrombosis and reocclusion. Dynamic measuement of TXB2 and GMP-140 might help to predict vascullar reocclusion and provide diagnostic values clinically.