植物激素对红花再生体系的影响(英文)

The Effect of Phytohormones on Safflower Regeneration Plant

目的:研究植物激素对红花再生植株的影响,为红花的良种快速繁殖、遗传转化等研究提供基础。方法:以红花无菌苗的子叶作为外植体,研究了植物激素对红花愈伤组织诱导、分化和生根的影响。结果:1.不同激素单独使用均能诱导出愈伤组织,但没有直接产生不定芽;2.不同植物激素配合使用对不定芽的分化率明显不同,生根情况也存在差异显著;3.子叶愈伤组织诱导培养基:MS+3%蔗糖+0.7%琼脂+1.0 mg/L NAA;分化培养基:MS+3%蔗糖+0.7%琼脂+0.2 mg/L NAA+1.0 mg/L6-BA;生根培养基:1/4 MS+2.0 mg/L NAA+0.5mg/L IAA。结论:通过单一激素与两激素配合使用,筛选出愈伤组织诱导、分化和组培苗生根的最佳培养基。

Objective ： To study the effect of phytohormones on the regeneration of safflower, in order to provide a foundation for the rapid propagation of safflower seeds and genetic transformation. Methods： The experiment used the cotyledons as the explant and studied the influence of calli induction, differentiation and rooting by phytohormones. Results： 1. Different hormones by single using induced calli, but didn＇t directly produce adventitious buds; 2. With the combination of different phytohormones on the differentiation of adventitious buds were significantly different, the roots were also significantly different; 3. Cotyledon calli induction medium： MS ＋ 3% Sucrose ＋0.7% Agar ＋ 1.0 mg/L NAA; differentiation medium： MS ＋3% Sucrose ＋0.7% Agar ＋0.2 mg/L NAA ＋ 1. 0 mg/L 6-BA; rooting medium： 1/4 MS ＋ 2. 0 mg/L NAA ＋0. 5 mg/L IAA. Conclusion： We have screened out the best culture medium of calli induction, differentiation and rooting via using singularity phytohormone and two phytohormones.