罗氏海盘车粘多糖的提取及其体外免疫调节作用

Extracts of Mucopolysaccharides from Asterias rollestoni and Its Immunoregulation Effect in vitro

目的：从罗氏海盘车体壁、内脏中分别提取粘多糖MP1和MP2，测定其对正常小鼠脾细胞及皂苷作用后的脾细胞免疫活性的影响。方法：罗氏海盘车内脏先经丙酮脱脂，根据正交实验分析确定最佳热水提取条件，然后经热水提取、去蛋白、醇沉得内脏粘多糖MP2。小鼠脾细胞体外培养并分为5组：MP1组、MP2组、皂苷组、MP1＋皂苷组及MP2＋皂苷组，分别加入相应药物，MTT法分别测定对脾细胞的体外增殖影响。结果：不同浓度MP1和MP2对脾细胞均有一定的促进增殖作用，该作用随多糖剂量的升高先增高后降低；并不同程度地抑制皂苷的细胞毒作用，终浓度为25～40mg／mL的MP2能逆转皂苷的细胞毒作用，其量效关系是以40mg／mL（P〈0．01）为顶点的抛物线。结论：MP1和MP2均对正常小鼠的免疫功能有促进作用，且以后者效果明显；MP2具有显著的逆转皂苷脾细胞毒作用。

Objective：To study immunoregulation effects on normal pulpal cells of the mucopolysaccharides MP1 and MP2 extracted from Asterias rollestoni body wall and viscera, respectively. Methods ： The orthogonal design was employed to obtain the best possible combination of the critical parameters for mucopolysaccharide MP2. MP1, MP2, glycosides, MP1 ＋ glycosides and MP2 ＋ glycosides were added respectively into pulpal cells culture media to test their effects on spleen cells proliferations by MTr assay. Resuhs：MP1 and MP2 could certainly increase the proliferation of the normal splenocyte and inhibit glycosides cytotoxicity. Compared with controls, MP2 reversed the cytotoxicity of glycosides at concentration of 25 to 40 mg/mL, the great est activity appearing at the dose of 40 mg/mL （P 〈0.01 ） ,while MP1 didn＇t reverse it. Conclusion：MP1 and MP2 can enhance immunity of normal mice,and MP2 can significantly reverses the cytotoxicity of glycosides in vitro.