摘要
1材料与方法
1．1试验材料与DNA提取2007年，在江苏省苏州市种羊场随机选取成年羊88只，采取耳组织提取DNA，-20℃保存。并记录湖羊的初生重、断奶重和6月龄重。
[ Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. [ Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level （PIC 〉0.5）. [ Conclusion ] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.
基金
Supported by the State Scientific Basic Research Platform Program( No. 2005DKA21101)
China Postdoctoral Science Foundation(No. 20080430470)
Natural Science Foundation of Jiangsu Prov-ince of China (BK2007556)
the National high-tech R&D program(863 program)(No.2006AA10Z198)
Key Projects in the NationalScience &Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2006BAD13B08 )
Support Foundation of Chinaduring the 11th Five-Year Plan Period (No. 2008BADB2B04)
BasicNatural Science Foundation for Colleges and Universities in JiangsuProvince (NK051039)
Jiangsu Government Scholarship for Over-seas Studies Project
Qing Lan Project of Colleges and UniversitiesJiangsu Province
the New Century Talent Project of Yangzhou University in China~~
