摘要
1材料与方法
1．1材料番茄黄化曲叶病毒（TYLCV）是由烟粉虱以持久方式传播，机械接种传毒，但较困难；种子不带毒，植株间接触也不传毒，所以该试验依靠烟粉虱传播使材料获得病毒表现症状。毒原为上海农科院园艺所保持的带有TYLCV的烟粉虱。2006年7月中旬将番茄材料播种在50孔的穴盘中，以草炭为基质，8月中旬定植在具有足够传播TYLCV烟粉虱的玻璃温室内，每天间断性的驱赶，使烟粉虱充分接触试验材料。供试番茄是对TYLCV的感性品种“2300”，健康植株品种“2300”定植在没有毒源的温室内为对照。
[Objective] The aim was to study the effect of tomato yellow leaf curl virus （TYLCV） infection on leaf anatomical structure and protective enzyme system of tomato. [Method] The anatomical structure of infected and healthy leaves of tomato were observed and compared by using paraffin section method. The activity changes of SOD, POD and CAT in the infected leaves of tomato were determined. [ Result] The results revealed that there were some differences in anatomical structure between healthy and infected leaves. Some cells of infected leaves were damaged so that the leaves curled and became yellow, which affected the normal function of organs. Compared with control, enzyme activities in the tomato plants infected by TYLCV were enhanced at the early periods and higher than that in control, then started to decline at the middle and late periods but lower than that in control.[ Conclusion] After infection by TYLCV, the leaf anatomical structure of tomato was changed greatly and the protective enzyme system was damaged severely, and affected the normal physJological metabolic functions of tissues and organs in tomato in further.
基金
Supported by the National 863 Program:Gene Polymerization Tech-nology Study and New Variety Breeding of High-quality
Multi-resist-ance and High-yield Tomato(2007AA10Z178)
Shanghai Agricul-ture Committee Key Project
Germplasm Innovation of Tomato Re-sistance to Yellow Leaf Curl Virus(2007)~~
