胶体金免疫层析法快速检测食品中苏丹红I残留

Development of a one-step immunochromatographic test strip for rapid detection of Sudan I residue in food

通过设计合成苏丹红I衍生物,将其与明胶用混合酸酐法合成苏丹红I-明胶免疫抗原,免疫新西兰白兔制备抗苏丹红I多克隆抗体。采用柠檬酸三钠还原法制备颗粒大小为24.5 nm的胶体金,并制备苏丹红I-BSA抗原-胶体金复合物,组装检测试纸。实验结果表明,试纸条法的苏丹红I检测线为15μg/kg,抗苏丹红I抗体与苏丹红Ⅱ、Ⅲ、Ⅳ均无交叉反应性,与高效液相测定差异不显著。检测时间5min,适用于食品中苏丹红I残留的快速检测。

A one - step immunochromatographic （IC） test strip for rapid detection of Sudan Ⅰwas developed. Sudan Ⅰ- gelatin immunogen was obtained via mixed acid anhydride method to couple Sudan Ⅰderivative with gelatin. The immunoantigen was injected into new zealand white rabbit to prepare the anti - Sudan Ⅰpolyelonal antibody. The 24.5 nm colloidal gold particles were prepared with the method of trisodium citrate reduction. Then the Sudan Ⅰ- BSA antigen - colloidal gold compounds were made for test strip preparation. The results showed that the detection limit of the test strip was 15 μg/kg. There was no cross - reactivity was detected between Sudan Ⅰ antibody and SudanⅡ, Sudan Ⅲ or Sudan IV. The results demonstrated there was no significant different between the results of IC strip and HPLC detection. With IC test strip, the Sudan Ⅰresidue in food could be rapidly detected within 5 min, which was suitable for rapid testing on - site.