安徽合肥地区汉族群体HLA基因频率分布

Analysis and Significance of HLA Polymorphism in Hefei Anhui Individuals of Han Nationality

目的分析安徽合肥地区汉族造血干细胞捐献者人类白细胞抗原HLA-A,B,DRB1基因频率的分布特征。方法应用PCR-SSOP和PCR-SSP基因分型技术,对合肥地区1174名造血干细胞捐献者血样进行HLA-A,B,DRB1基因分型,并用统计学方法对基因型频率进行Hardy-Weinberg平衡检验。结果共检出61个基因,合肥地区HLA-A,B,DRB1位点基因频率最高的分别为A*02(37.3%),A*11(20.8%),A*24(17.8%);B*15(13.8%),B*40(17.6%),B*13(11.3%);DRB1*15(16%),DRB1*09(15%),DRB1*04(14%)。发现了A*34和B*15中的B*95等在中国人群中比较罕见的基因。结论合肥地区汉族造血干细胞捐献者HLA部分位点的基因频率呈现出地区差异。

Objective To study the polymorphism of HLA antigen in Anhui Han nationality. Methods PCR-SSOP and PCR-SSP were used to determine HLA-A,B,DRB1 alleles in 1 174 unrelated healthy Han individuals of Hefei,Anhui. Results 61 alleles were detected in our study. Amone these alleles the highest alleles frequencies of HLA-A,B,DRB1 were A ＊ 02（37.3%）,A ＊ 11（20.8%）,A ＊ 24（17.8%）;B ＊ 15（13.8%）,B ＊ 40（17.6%）,B＊ 13（11.3%）;DRB1 ＊ 15（16%）, DRB1 ＊ 09 （15 % ）,DRB1 ＊ 04 （14% ）. And several scarce alleles such as A ＊ 34,B ＊ 95 were discovered in our study. Conclusion There is obvious regional difference in some of HLA alleles frequencies of Hefei Han population.